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In contrast to random primers, oligo (dT) primers are usually not suited to degraded samples but are exceptional for setting up cDNA libraries and advised for use with reverse transcriptases (RTs) as Superscript IV RT.

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Contrary to oligo(dT) primers, these primers will not be template certain and they are suitable for DNA synthesis utilizing Klenow fragments with DNA templates or for cDNA synthesis applying reverse transcriptase with mRNA templates.

Swimming pools of pre-developed primers may help generate thorough expression profiles, Specially exactly where assay sensitivity is critically crucial.

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